Refractometers sold for the aquarium hobby are meant to measure the refraction of NaCl, not seawater. Apparently there is a difference. Plus, they're pretty inconsistent in terms of quality control. There are all sorts of stories about varying results with them. Randy Holmes-Farley wrote a coupe of good articles about this. You can use a specific pinpoint conductivity calibration fluid to calibrate hobby level refractometers at 35PPT, which is the range they need to be accurate in. Calibrating with R.O. water only insures that they'll read zero PPT correctly.
Maybe the easiest cheapest answer is to have a big glass floating hydrometer and an accurate thermometer, or use the pinpoint solution to calibrate the refractometer. as several people have pointed out, there's an acceptable salinity range, so being exactly at 35PPT is not so important.



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