Here a a few thoughts I had while reading this article. Again, I am not discounting what is being theorized, but I'd like to see a broader study done.
1) "To date, the cause(s) of this syndrome have not been identified in aquarium fish through published scientific study, although conjecture and informal studies abound." They admit from the get-go that there still isn't a concensus as to the cause of hlle.
2) "When the survey results were limited to professional public aquarists, 19% of the respondents found complete reversal of HLLE by discontinuing the use of carbon filtration." So 100 "advanced" aquarists were surveyed. There were 18 treatment methods shown to have worked, 84% of those methods involved moving the fish to new environments, and only 19% saw a reversal of hlle when carbon was discontinued? The number of people keeps getting watered down, while the variables keep going up. Mathematically, this doesn't prove a thing to me.
3) "Three 120 gallon marine aquarium systems (two tanks per system) were established using typical home aquarium equipment and synthetic seawater. Live rock was utilized as the basis for biological filtration in all three systems. Thirty-five Ocean Surgeonfish, (Acanthurus bahianus) were evenly distributed among the three systems at the start of the study." This does not constitute a comprehensive study IMHO. A much broader study conducted in numerous settings around the country done by different experts would be needed for a more complete study. The article does state that other public aquariums have reported similar findings, but no information was given about them. This seems like too narrow of a snapshot to tell everyone to start dumping their carbon in the trashcan. Plus, a more comprehensive peer review done of a longer period of time would be needed.
4) "It has also been reported that aquariums which use foam fractionators (protein skimmers) do not seem to develop HLLE as frequently, even when carbon is routinely used." I'd be willing to bet most systems in this hobby use a skimmer. When I got into reefing 15 years ago, my first two purchases were in this order; a fish tank, a skimmer. Wouldn't this mitigate most of the threats of from carbon in the majority of systems?
5) "In addition, the hard pelleted carbon used in this study did not cause severe HLLE, while the soft, dusty carbon did." I have yet to see one reefer out there that does not use pellatized carbon. The article even states that most aquarist do not use the dusty type of carbon. Plus, I have yet to see one box, bag, or container of carbon that does not specifically state to completely rinse your carbon before using it in a tank.
The new article does not change my opinion from last year. It's basically the same thing as the previous write-up, but with an update on the results from the Toledo Zoo experiment. The people there did a GREAT job of using a controlled setup, but I still think there's too many factors, and too little time researching this for me to throw my container of carbon in the trashcan.
I refuse to join any club that would have me as a member.
Groucho Marx